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. 2021 Sep 16;22(18):10025. doi: 10.3390/ijms221810025

Figure 4.

Figure 4

Effect of RNA-PPRH on survivin mRNA and protein levels in PC-3 cells. (A) Effect of RNA-PPRH on survivin mRNA levels. PC-3 cells (30000) were transfected with 100 nM of a DNA-PPRH negative control HpSC4, 100 nM of the DNA-PPRH HpsPr-C (HpDNA), 800 nM of HpsPr-C-RNA (HpRNA), or 5 µg of pPPRH-HpsPr-C (pPPRH). Then, 24 h after transfection, or 48 h in the case of pPPRH, survivin mRNA levels were determined by RT-qPCR. TATA-binding protein (TBP) was used to normalize the results. (B) Effect of RNA-PPRH on survivin protein levels. PC-3 cells (30000) were treated 24 h with 800 nM of the specific RNA-PPRH HpsPr-C (HpRNA) or 48 h with 5 µg of pPPRH-HpsPr-C (pPPRH); then, protein extracts were obtained to analyze survivin protein levels. (C) Representative images of Western blots. Tubulin protein levels were used to normalize the results. (D) Fluorescence microscopy images of each condition were taken before analysis. Data represent the mean ± SEM from three experiments. Statistical significance was determined using a one-way ANOVA with Tukey’s multiple comparisons test (* p < 0.05, ** p < 0.01, **** p < 0.0001).