Figure 6.

Effect of Adv-PPRH on survivin mRNA and protein levels in HeLa cells. HeLa cells (15,000) were plated the day before infection (100 MOI). mRNA levels (A) and protein levels (B) were analyzed 72 h after infection. Survivin mRNA levels were determined by RT-qPCR. TATA-binding protein (TBP) was used to normalize the results. (C) Representative images of Western blots are shown. Tubulin protein levels were used to normalize the results. (D) Fluorescence microscopy images of each condition were taken before each analysis. Error bars represent the standard error of the mean of three experiments. Statistical significance was calculated using one-way ANOVA with Dunnett’s multiple comparisons test (* p < 0.05, ** p < 0.01). Abbreviation: CNT—control.