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. 2021 Aug 24;13(9):2910. doi: 10.3390/nu13092910

Figure 1.

Figure 1

Vitamin D/Vitamin D receptor alleviates IR-induced DNA damage and apoptosis in IEC-6 cells. (A) Representative immunofluorescence (IF) images of γH2AX staining in IEC-6 cells at 1 h post 12 Gy IR pretreated 10 nM 1,25(OH)2D3. (B) Quantification of percentage of γH2AX+ cells was analyzed. (C) Representative IF images of Annexin V/PI staining in IEC-6 cells at 24 h post 12 Gy IR pretreated 10 nM 1,25(OH)2D3. (D) Quantification of apoptotic cells with Annexin V-positive signaling was analyzed. # Compared with the 0 Gy-control group; * compared with the 12Gy-VD group. (E) Vitamin D receptor (VDR) and Cleaved Caspase 3 (Casp3) in IEC-6 cells were determined by WB at 24 h after 12 Gy IR pretreated with 10 nM 1,25(OH)2D3 for 6 h. Quantitative analysis of Western blotting of VDR and Cleaved Casp3 expression. Values are means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001; ## p < 0.01, ### p < 0.001. Scale bar, 20 μm.