KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-CD127 (clone: A7R34) | Biolegend | Cat:135024; RRID:AB_11218800 |
| Anti-CD127 (clone: A7R34) | Biolegend | Cat:135037; RRID:AB_2565269 |
| Anti-CD4 (clone: RM4-5) | Biolegend | Cat:100542; RRID:AB_2563051 |
| Anti-CD62L (clone: MEL-14) | Biolegend | Cat:104440; RRID:AB_2629685 |
| Anti-CD62L (clone: MEL-14) | BD | Cat: 612833; RRID:AB_2870155 |
| Anti-T-bet (clone: 4B10) | Biolegend | Cat:644820; RRID:AB_2715766 |
| Anti-PD-1 (clone: 29F.1A12) | Biolegend | Cat:135220; RRID:AB_2562616 |
| Anti-CD244.2(2B4) (clone: m2B4(B6)458.1) | Biolegend | Cat:133504; RRID:AB_1595642 |
| Anti-CD319 (slamf7) (clone: 4G2) | BD | Cat:747995; RRID:AB_2872456 |
| Anti-CD44 (clone: IM7) | Biolegend | Cat:103032; RRID:AB_2076204 |
| Anti-CD11a (clone: M17/4) | BD | Cat:740676; RRID:AB_2740363 |
| Anti-NK1.1 (clone: PK136) | BD | Cat:746876; RRID:AB_2871676 |
| Anti-γδTCR (clone: GL3) | BD | Cat:746962; RRID:AB_2871745 |
| Anti-NKG2A/C/E (clone: 20d5) | Invitrogen | Cat:46-5896-82; RRID:AB_10853352 |
| Anti-CD226 (clone: 10E5) | Biolegend | Cat:128806; RRID:AB_1186119 |
| Anti-NKG2D (clone: CX5) | Biolegend | Cat:130214; RRID:AB_2728148 |
| Anti-c-Maf (clone: sym0F1) | Invitrogen | Cat:25-9855-80; RRID:AB_2811794 |
| Anti-CD3e (clone: 145-2C11) | Biolegend | Cat:100310; RRID:AB_312675 |
| Anti-CD3 (clone: 17A2) | Biolegend | Cat: 100234; RRID:AB_2562555 |
| Anti-CD39 (clone: 24DMS1) | Invitrogen | Cat:25-0391-80; RRID:AB_1210767 |
| Anti-CD8a (clone: 53-6.7) | Biolegend | Cat:100730; RRID:AB_493703 |
| Anti-CD8a (clone: 53-6.7) | Invitrogen | Cat:46-0081-82; RRID:AB_1834433 |
| Anti-CD366 (clone: Tim-3) | Biolegend | Cat:119738; RRID:AB_2810368 |
| Anti-TCF1/7 (clone: S33-966) | BD | Cat:564217; RRID:AB_2687845 |
| Anti-CD45 (clone: 30-F11) | Biolegend | Cat:103124; RRID:AB_493533 |
| Anti-Eomes (clone: Dan11mag) | Invitrogen | Cat:53-4875-82; RRID:AB_10854265 |
| Anti-Eomes (clone: Dan11mag) | Invitrogen | Cat:15-4875-82; RRID:AB_2802207 |
| Anti-KLRG1(clone: 2F1) | Invitrogen | Cat:48-5893-82; RRID:AB_10852843 |
| Anti-CD223(LAG-3) (clone: C9B7W) | Biolegend | Cat:125224; RRID:AB_2572082 |
| Anti-TNFa (clone: MP6-XT22) | Biolegend | Cat:506339; RRID:AB_2563127 |
| Anti-IL-2 (clone: JES6-5H4) | BD | Cat: 566363; RRID:AB_2739711 |
| Anti-IFNg (clone: XMG1.2) | Biolegend | Cat: 505838; RRID:AB_2629667 |
| TB10.4 Tetramer | NIH | N/A |
| 32ATetramer | NIH | N/A |
| Bacterial and virus strains | ||
| Erdman | Grew up in house | N/A |
| H37Rv | Grew up in house | N/A |
| CDC1551 | Dr. Christopher Sassetti | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| RNAprotect Cell reagent | QIAGEN | Cat:76526 |
| Critical commercial assays | ||
| Foxp3/transcription factor staining buffer set | Invitrogen | Cat:00-5523-00 |
| CD8 T cell isolation kit | Miltenyi | Cat:130-117-044 |
| LEGENDplex™ | Biolegend | Customized item |
| RNeasy mini kit | QIAGEN | Cat:74104 |
| Zombie Aqua fixable viability kit | Biolegend | Cat: 423102 |
| Zombie UV fixable viability kit | Biolegend | Cat: 423017 |
| Deposited data | ||
| Comparison of WT versus MHCII KO CD8 T cell transcriptomes from lungs of Mycobacterium tuberculosis infected mice | This paper | GEO: GSE167439 |
| Experimental models: Organisms/strains | ||
| P25 TCRtg | Bred in house | Stock# 011005 |
| MHCII KO | The Jackson Laboratory | Stock# 003584 |
| TCRa KO | The Jackson Laboratory | Stock# 002116 |
| KbDb | Dr. Kenneth Rock | Stock# 019995 |
| C57BL/6J | The Jackson Laboratory | Stock# 000664 |
| Software and algorithms | ||
| FlowJo v10.7.1 | Flowjo | N/A |
| GraphPad Prism | GraphPad | N/A |
| GSEA | The Broad Institute | https://www.gsea-msigdb.org/gsea/index.jsp |
| clusterProfiler (v3.14.3) | Bioconductor | https://bioconductor.org/packages/release/bioc/html/clusterProfiler.html |
| Pheatmap | CRAN | https://cran.r-project.org/web/packages/pheatmap/index.html |