Figure 2.

C5aR1 antagonist or rGCase effectively prevent excess secretion of TNF-α. GD macrophages were stimulated with rC5a (3 nM) in the presence or absence of PMX53 (50 nM) at 37 °C for 8 h. Recombinant GCase (0.24 U/mL) was added to the media every 24 h for 3 days prior to rC5a stimulation. The supernatant was collected and quantified for the expression of TNF-α by ELISA. Results are represented as the mean (n = 3; *** p < 0.001, **** p < 0.0001).