Figure 5.

qRT-PCR validation of select genes from the gene array. qRT-PCR validation of genes selected from the microarray profile. Total RNA from the macrophage preparations was analyzed by qRT-PCR for the expression of a subset of genes randomly selected from those up- or down- regulated genes after 4 h of incubation with rC5a (30 nM). Expression changes were standardized to GAPDH. Results are presented as relative gene expression. (n = 3; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).