FIG. 7.

Northern blot analysis of TCA cycle gene expression. Total RNA was prepared from PSY142 [rho⁺] and [rho⁰] wild-type (WT) strains and their rtg1Δ, rtg2Δ, and rtg3Δ derivatives grown to mid-log phase on YPR medium. Blots were probed for each of the indicated genes as described in Materials and Methods. RNA loads were normalized to the level of transcripts of the ACT1 gene. CIT2 transcripts were also analyzed as a control.