Correlation of microsatellite instability (MSI) site percentage with tumor mutation burden (TMB) and PD-L1 expression. MSI site percentage showed a significant correlation with (A) TMB, (B) PD-L1 CPS score, and (C) PD-L1 TPS score in 588 cases. The MSI site percentage was significantly correlated with the PD-L1 CPS (D) (p = 0.014, R2 = 0.081), but not with PD-L1 TPS (E) in biliary tract carcinoma (p = 0.118). In gastric carcinoma, the MSI site percentage was significantly correlated with PD-L1 CPS score (F) and PD-L1 TPS score (G) (p < 0.001 and R2 = 0.291; p < 0.001 and R2 = 0.506, respectively).