Skip to main content
. 2021 Sep 15;11(9):1364. doi: 10.3390/biom11091364

Figure 3.

Figure 3

sCT S29 to hydroxyproline mutation markedly decreased sCT(22–32) affinity for CTR ECD. (a) The hypothetical structure of CTR ECD (PDB 6PFO, Mol A) with sCT(22–32) N26D/S29HYP mutations. CTR ECD H121, E123, and N124 and sCT N26D and S29HYP mutated residues were shown both with stick and surface representations. The distance between sCT HYP29 and CTR ECD E123 was shown as a dotted red line and was measured as 1.0 Å. (b) FP competition peptide binding assay with sCT(22–32) N26D/S29P and N26D/S29HYP mutations. FITC-sCT(22–32) was used as a peptide probe. Representative peptide-binding curves were shown from three independent experiments.