Table 1.
Intra-assay Percent Coefficient of Variance (CV%) within Metabolite Classes and CV% Standard Deviation (SD) for Technical Replicates of PTSD and Control Samples in Shipment 1.
| Intra-Assay Precision: Shipment 1 | |||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| PTSD Average CV% | Control Average CV% | Count | Range | SD | PTSD Average CV% | Control Average CV% | Count | SD | PTSD Average CV% | Control Average CV% | Count | SD | PTSD Average CV% | Control Average CV% | Count | SD | PTSD Average CV% | Control Average CV% | Count | SD | |
| Metabolite Class | Biocrates | HMT | Nightingale | Lipotype | Metabolon | ||||||||||||||||
| Acylcarnitines | 10.33 | 9.88 | 14 | 2.07–76.09 | 15.67 | 5.66 | 5.81 | 35 | 7.43 | 9.03 | 8.39 | 21 | 7.70 | ||||||||
| Amino Acids | 3.53 | 8.12 | 40 | 0.87–19.01 | 3.14 | 6.85 | 6.93 | 22 | 2.56 | 2.78 | 6.01 | 9 | 3.76 | 8.84 | 9.56 | 52 | 8.22 | ||||
| Amino Acid Related | 3.44 | 8.71 | 1.73–12.88 | 3.21 | |||||||||||||||||
| Carboxylic Acids | 3.85 | 7.20 | 2 | 2.70–8.02 | 2.25 | 5.10 | 5.62 | 8 | 2.83 | 2.99 | 6.66 | 3 | 2.38 | 11.32 | 9.13 | 101 | 13.59 | ||||
| Cholesteryl ester | 8.46 | 14.69 | 18 | 3.42–48.13 | 8.44 | 4.23 | 13.30 | 13 | 6.84 | 4.63 | 4.00 | 26 | 2.20 | ||||||||
| Diglycerides | 8.46 | 12.28 | 14 | 1.95–36.34 | 7.53 | 4.84 | 9.80 | 2 | 3.38 | 4.29 | 4.00 | 19 | 2.73 | ||||||||
| Diazines | 21.00 | 18.45 | 3 | 21.16 | 15.39 | 13.46 | 5 | 12.60 | |||||||||||||
| Organonitrogen compounds | 7.29 | 9.37 | 13 | 5.02 | 15.55 | 11.3 | 14 | 12.61 | |||||||||||||
| Purine nucleotides | 9.69 | 16.16 | 5 | 9.32 | 14.18 | 12.48 | 10 | 8.09 | |||||||||||||
| Organooxygen compounds | 12.03 | 9.63 | 6 | 7.35 | 1.04 | 6.44 | 2 | 4.07 | 9.19 | 9.90 | 6 | 8.38 | |||||||||
| Hydroxy acids and derivatives | 4.04 | 6.79 | 5 | 2.47 | 0.88 | 3.05 | 1 | NA | 11.28 | 11.80 | 16 | 10.81 | |||||||||
| Keto acids and derivatives | 1.62 | 5.52 | 3 | 2.24 | 4.29 | 26.20 | 2 | 18.87 | 8.35 | 8.18 | 13 | 4.94 | |||||||||
| Ceramides | 6.53 | 8.60 | 25 | 0.59–29.33 | 5.33 | 11.36 | 10.43 | 5 | 4.62 | 5.03 | 6.77 | 2 | 3.06 | 7.22 | 7.10 | 11 | 5.55 | ||||
| Lactosylceramide | 19.98 | 15.25 | 13 | 13.62 | 10.15 | 14.99 | 12 | 10.36 | |||||||||||||
| Glucosylceramide | 21.84 | 19.55 | 13 | 11.15 | |||||||||||||||||
| Dihexosylceramides | 10.30 | 9.78 | 10 | 2.45–34.65 | 7.59 | ||||||||||||||||
| Trihexosylceramides | 14.16 | 17.94 | 6 | 4.69–43.82 | 11.60 | ||||||||||||||||
| Dihydroceramide | 19.07 | 17.15 | 12 | 14.73 | |||||||||||||||||
| Hexosylceramide | 9.56 | 11.32 | 19 | 1.33–27.20 | 5.81 | 6.28 | 8.33 | 12 | 4.34 | ||||||||||||
| Triglycerides | 7.33 | 13.27 | 235 | 0.78–38.42 | 3.72 | 4.88 | 6.49 | 32 | 2.46 | 1.92 | 5.96 | 21 | 2.19 | ||||||||
| Hormones/Steroids | 2.53 | 6.43 | 3 | 1.48–7.15 | 2.34 | 5.88 | 6.28 | 9 | 4.77 | 8.65 | 6.18 | 26 | 3.79 | ||||||||
| Fatty Acids | 8.10 | 8.16 | 7 | 3.63–11.43 | 2.60 | 6.92 | 9.11 | 32 | 4.97 | 27.57 | 3.89 | 2 | 15.39 | 3.58 | 3.53 | 29 | 2.15 | ||||
| Fatty Acyls | 28.92 | 29.75 | 4 | 19.76 | 18.17 | 13.20 | 83 | 12.09 | |||||||||||||
| Biogenic Amines | 4.74 | 10.52 | 3 | 3.19–11.69 | 3.47 | ||||||||||||||||
| Bile Acids | 5.84 | 7.73 | 12 | 2.48–12.04 | 2.77 | 4.66 | 6.47 | 4 | 7.22 | 11.42 | 8.94 | 22 | 6.95 | ||||||||
| Indoles and Derivatives | 3.36 | 10.40 | 3 | 2.69–14.21 | 4.56 | 6.51 | 7.37 | 1 | NA | 7.45 | 5.82 | 9 | 3.97 | ||||||||
| Lysophosphatidyl-cholines (LPC) | 13.05 | 11.32 | 14 | 0.91–36.05 | 10.66 | 3.34 | 3.71 | 27 | 1.69 | 4.38 | 14.44 | 6 | 5.36 | 7.28 | 10.10 | 15 | 5.14 | ||||
| Glycerophosphocholines | 7.09 | 7.76 | 19 | 10.79 | |||||||||||||||||
| Phosphatidyl-cholines (PC) | 7.98 | 9.78 | 73 | 1.78–67.84 | 9.56 | 7.96 | 11.15 | 63 | 5.22 | 8.12 | 5.98 | 18 | 4.44 | ||||||||
| Sphingomyelins | 3.89 | 7.68 | 15 | 1.88–11.73 | 2.64 | 5.79 | 8.62 | 12 | 2.89 | 3.45 | 3.05 | 12 | 1.79 | ||||||||
| Sphingolipids | 14.28 | 13.47 | 12 | 10.86 | 6.13 | 7.79 | 2 | 2.11 | |||||||||||||
| Sphinganine | 6 | ||||||||||||||||||||
| Sphingosine | 11.76 | 12.89 | 6 | 9.48 | |||||||||||||||||
| Glycerophospholipids | 10.41 | 9.67 | 15 | 8.70 | 19.61 | 22.64 | 7 | 16.45 | |||||||||||||
| Glycerolipids (Monoacylglycerol) | 21.69 | 26.95 | 25 | 14.48 | |||||||||||||||||
| Carboximidic acids and derivatives | 4.93 | 4.33 | 1 | NA | 15.21 | 17.34 | 4 | 10.22 | |||||||||||||
| lyso-Phosphatidylethanolamine (LPE) | 10.36 | 8.40 | 22 | 7.95 | 8.83 | 15.24 | 9 | 5.24 | 5.18 | 6.38 | 8 | 4.88 | |||||||||
| Phosphatidylcholine (-ether) (LPC-O) | 13.02 | 16.40 | 41 | 8.64 | |||||||||||||||||
| Phosphatidylethanolamine (PE) | 9.22 | 15.06 | 15 | 6.79 | 4.38 | 8.11 | 12 | 6.46 | |||||||||||||
| Phosphatidylethanolamine (-ether) (LPE-O) | 11.54 | 15.20 | 16 | 7.76 | |||||||||||||||||
| Phosphatidylinositol (LPI) | 7.37 | 8.44 | 14 | 6.05 | 8.95 | 16.88 | 15 | 7.62 | 10.83 | 18.82 | 6 | 8.90 | |||||||||
| Lyso-Phosphatidylserine (LPS) | 11.21 | 15.84 | 7 | 8.97 | |||||||||||||||||
| Glycerophosphoglycerols (LPG) | 9.23 | 10.92 | 14 | 6.71 | |||||||||||||||||
| Vitamins and Cofactors | 1.09 | 10.44 | 1 | NA | 2.37 | 5.87 | 1 | NA | |||||||||||||
| Alkaloids | 4.69 | 13.74 | 1 | NA | 3.86 | 2.48 | 2 | 0.80 | |||||||||||||
| Amine (Oxides) | 6.92 | 8.23 | 1 | NA | 45.46 | 12.49 | 1 | NA | |||||||||||||
| Carbohydrates and Related | 2.50 | 6.33 | 1 | NA | 10.81 | 14.37 | 34 | 13.62 | |||||||||||||
| Cresols | 2.07 | 7.14 | 1 | NA | |||||||||||||||||
| Imidazopyrimidines | 11.35 | 5.44 | 1 | NA | 10.32 | 7.60 | 17 | 8.68 | |||||||||||||
| 5′-deoxyribonucleosides | 19.82 | 18.62 | 1 | NA | 7.84 | 8.63 | 1 | NA | |||||||||||||
| Nucleoside and nucleotide analogues | 13.05 | 3.4 | 1 | NA | |||||||||||||||||
| Pyrimidine nucleosides | 1.33 | 7.94 | 1 | NA | 9.24 | 9.59 | 7 | 7.33 | |||||||||||||
| Pyridines and derivatives | 6.69 | 7.90 | 10 | 5.76 | |||||||||||||||||
| Quinolines and derivatives | 11.85 | 13.64 | 3 | 7.42 | |||||||||||||||||
| Phenols | 9.22 | 4.79 | 3 | 5.40 | |||||||||||||||||
| Prenol lipids | 17.87 | 8.77 | 7 | 8.13 | |||||||||||||||||
| Imidazole ribonucleosides and ribonucleotides | 12.17 | 5.5 | 1 | NA | |||||||||||||||||
| Benzene and substituted derivatives | 12.72 | 9.49 | 14 | 10.36 | |||||||||||||||||
| Phenylpropanoic acids | 6.52 | 5.82 | 9 | 8.63 | |||||||||||||||||
| Tetrapyrroles and derivatives | 3.45 | 11.6 | 2 | 5.43 | |||||||||||||||||
| Cholesterol and derivatives | 7.53 | 9.5 | 2 | 4.40 | |||||||||||||||||
| Non-metal oxoanionic compounds | 2.94 | 3.17 | 2 | 1.81 | |||||||||||||||||
| Organic sulfuric acids and derivatives | 6.1 | 4.03 | 22 | 3.49 | |||||||||||||||||
| Organic sulfonic acids and derivatives | 2.47 | 5.59 | 2 | 3.48 | |||||||||||||||||
| Organic carbonic acids and derivatives | 6.33 | 9.48 | 2 | 3.88 | |||||||||||||||||
| Organic phosphoric acids and derivatives | 9.53 | 7.87 | 1 | NA | |||||||||||||||||
| Benzothiazepines | 2.29 | 6.17 | 2 | 5.83 | |||||||||||||||||
| Bilirubins | 9.03 | 5.41 | 2 | 6.66 | |||||||||||||||||
| Dihydrofurans | 6.94 | 3.07 | 2 | 3.68 | |||||||||||||||||
| Alkyl halides | 2.9 | 3.98 | 2 | 1.49 | |||||||||||||||||
| Sulfinic acids and derivatives | 11.13 | 16.04 | 1 | NA | |||||||||||||||||
| Azoles | 11.11 | 10.6 | 7 | 5.90 | |||||||||||||||||
| Azolidines | 4.34 | 6.92 | 1 | NA | |||||||||||||||||
| Cinnamic acids and derivatives | 4.07 | 13.92 | 1 | NA | |||||||||||||||||
| Peptidomimetics | 20.02 | 14.52 | 1 | NA | |||||||||||||||||
| Piperidines | 14.81 | 16.27 | 1 | NA | |||||||||||||||||
| Pyrrolidines | 4.09 | 5.48 | 1 | NA | |||||||||||||||||
| Coumarins and derivatives | 0 | 14.1 | 1 | NA | |||||||||||||||||
Notes: “High” precision is shown in green (≤10%), “moderate” in yellow (10% < x < 20%), and “low” in red (≥20%).