Table 2.
Inter-Assay percent Coefficient of Variance (CV%) within Metabolite Classes for Technical Replicates of PTSD and Control Samples across Shipment 1 and Shipment 2.
| Inter-Assay Precision: Shipment 1 vs. Shipment 2 | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| PTSD Average CV% | Control Average CV% | Standard deviation (SD) | PTSD Average CV% | Control Average CV% | Standard deviation (SD) | PTSD Average CV% | Control Average CV% | Standard deviation (SD) | PTSD Average CV% | Control Average CV% | Standard deviation (SD) | PTSD Average CV% | Control Average CV% | Standard deviation (SD) | |
| Metabolite Class | Biocrates | HMT | Nightingale | Lipotype | Metabolon | ||||||||||
| Acylcarnitines | 7.21 | 11.48 | 2.78 | 12.21 | 12.10 | 8.64 | 15.27 | 13.52 | 9.93 | ||||||
| Amino Acids | 5.88 | 11.95 | 3.57 | 9.10 | 9.79 | 4.02 | 4.80 | 7.37 | 3.48 | 12.84 | 14.21 | 8.03 | |||
| Amino Acid Related | 7.63 | 13.90 | 6.86 | ||||||||||||
| Carboxylic Acids | 7.54 | 13.71 | 4.05 | 10.60 | 8.90 | 5.83 | 5.92 | 7.90 | 1.51 | 15.38 | 13.91 | 12.75 | |||
| Cholesteryl ester | 11.71 | 14.47 | 2.58 | 8.07 | 8.93 | 5.25 | 10.16 | 8.68 | 7.77 | ||||||
| Diglycerides | 17.42 | 22.27 | 10.62 | 9.13 | 13.25 | 6.27 | 10.97 | 10.06 | 4.53 | ||||||
| Diazines | 16.42 | 21.57 | 14.04 | 33.22 | 29.05 | 24.92 | |||||||||
| Organonitrogen compounds | 13.31 | 11.50 | 8.24 | 14.25 | 13.66 | 7.67 | |||||||||
| Purine nucleotides | 30.06 | 38.64 | 18.99 | 11.49 | 11.84 | 5.98 | |||||||||
| Organooxygen compounds | 31.07 | 37.08 | 13.17 | 4.89 | 6.94 | 2.62 | 31.34 | 26.85 | 28.72 | ||||||
| Hydroxy acids and derivatives | 10.88 | 11.10 | 6.05 | 1.88 | 3.57 | NA | 17.76 | 17.90 | 12.03 | ||||||
| Keto acids and derivatives | 14.10 | 16.66 | 2.83 | 10.58 | 19.49 | 9.58 | 15.64 | 13.55 | 8.45 | ||||||
| Ceramides | 16.04 | 19.50 | 8.65 | 11.34 | 15.68 | 4.34 | 6.58 | 6.47 | 0.78 | 10.10 | 7.88 | 4.44 | |||
| Lactosylceramide | 15.32 | 16.79 | 8.06 | 16.61 | 17.51 | 13.28 | |||||||||
| Glucosylceramide | 19.30 | 23.43 | 9.77 | ||||||||||||
| Dihexosylceramides | 11.99 | 17.82 | 5.18 | ||||||||||||
| Trihexosylceramides | 17.08 | 23.39 | 4.52 | ||||||||||||
| Dihydroceramide | 18.63 | 21.21 | 12.20 | ||||||||||||
| Hexosylceramide | 14.23 | 17.72 | 6.01 | 11.44 | 10.57 | 3.49 | |||||||||
| Triglycerides | 15.42 | 19.35 | 7.56 | 8.34 | 6.67 | 2.20 | 9.3 | 7.65 | 2.61 | ||||||
| Hormones/Steroids | 15.59 | 18.54 | 9.04 | 8.08 | 10.35 | 5.86 | 15.39 | 13.53 | 5.84 | ||||||
| Fatty Acids | 13.80 | 18.43 | 7.40 | 24.26 | 27.33 | 33.29 | 53.25 | 9.39 | 26.15 | 6.89 | 6.60 | 3.39 | |||
| Fatty Acyls | 4.21 | 4.99 | NA | 20.94 | 17.94 | 9.44 | |||||||||
| Biogenic Amines | 6.37 | 18.78 | 10.04 | ||||||||||||
| Bile Acids | 21.36 | 23.51 | 28.05 | 5.99 | 10.49 | 10.09 | 21.36 | 20.83 | 11.05 | ||||||
| Indoles and Derivatives | 8.60 | 15.22 | 4.59 | 13.11 | 12.47 | 5.93 | |||||||||
| Lysophosphatidyl-cholines (LPC) | 13.24 | 17.78 | 8.17 | 6.45 | 8.75 | 3.38 | 9.13 | 8.85 | 0.77 | 17.64 | 17.23 | 12.98 | |||
| Glycerophosphocholines | 6.56 | 7.21 | 2.76 | ||||||||||||
| Phosphatidyl-cholines (PC) | 10.11 | 14.43 | 6.97 | 10.24 | 13.18 | 10.69 | 13.65 | 13.98 | 13.68 | ||||||
| Sphingomyelins | 9.34 | 12.99 | 4.28 | 6.16 | 7.19 | 2.09 | 6.51 | 7.58 | 4.35 | ||||||
| Sphingolipids | 20.21 | 20.23 | 10.83 | 9.90 | 12.50 | 1.60 | |||||||||
| Sphinganine | |||||||||||||||
| Sphingosine | 19.34 | 19.49 | 10.01 | ||||||||||||
| Glycerophospholipids | 19.51 | 22.85 | 25.01 | 22.79 | 26.91 | 17.68 | |||||||||
| Glycerolipids (Monoacylglycerol) | 35.32 | 34.69 | 15.53 | ||||||||||||
| Carboximidic acids and derivatives | 23.26 | 3.55 | NA | 12.92 | 17.33 | 5.22 | |||||||||
| lyso-Phosphatidylethanolamine (LPE) | 11.88 | 13.87 | 17.60 | 9.92 | 9.85 | 1.89 | 22.80 | 19.85 | 12.54 | ||||||
| Phosphatidylcholine (-ether) (LPC-O) | 14.35 | 15.00 | 6.39 | ||||||||||||
| Phosphatidylethanolamine (PE) | 12.50 | 14.70 | 6.08 | 12.16 | 12.16 | 8.38 | |||||||||
| Phosphatidylethanolamine (-ether) (LPE-O) | 10.14 | 11.68 | 3.76 | ||||||||||||
| Phosphatidylinositol (LPI) | 9.81 | 10.24 | 6.62 | 13.27 | 13.27 | 7.59 | 39.03 | 49.61 | 11.64 | ||||||
| Lyso-Phosphatidylserine (LPS) | 19.00 | 20.64 | 7.74 | ||||||||||||
| Glycerophosphoglycerols (LPG) | 12.34 | 15.52 | 7.60 | ||||||||||||
| Vitamins and Cofactors | 7.59 | 13.77 | NA | 6.44 | 13.52 | NA | |||||||||
| Alkaloids | 17.05 | 46.83 | 21.81 | ||||||||||||
| Amine (Oxides) | 6.55 | 11.15 | NA | 28.84 | 24.42 | NA | |||||||||
| Carbohydrates and Related | 6.61 | 13.25 | NA | 23.31 | 22.25 | 22.84 | |||||||||
| Cresols | 4.39 | 11.88 | NA | ||||||||||||
| Imidazopyrimidines | 19.81 | 26.41 | 14.25 | ||||||||||||
| 5′-deoxyribonucleosides | 12.33 | 9.07 | 5.31 | 10.04 | 13.25 | NA | |||||||||
| Nucleoside and nucleotide analogues | 44.29 | 15.88 | NA | ||||||||||||
| Pyrimidine nucleosides | 21.49 | 19.06 | 15.04 | ||||||||||||
| Pyridines and derivatives | 11.51 | 11.75 | 5.71 | ||||||||||||
| Quinolines and derivatives | 20.42 | 20.85 | 13.19 | ||||||||||||
| Phenols | 22.06 | 21.35 | 6.87 | ||||||||||||
| Prenol lipids | 16.43 | 13.54 | 6.28 | ||||||||||||
| Imidazole ribonucleosides and ribonucleotides | 7.24 | 7.26 | NA | ||||||||||||
| Benzene and substituted derivatives | 23.37 | 23.26 | 13.29 | ||||||||||||
| Phenylpropanoic acids | 17.47 | 23.77 | 12.32 | ||||||||||||
| Tetrapyrroles and derivatives | 9.73 | 17.57 | 5.40 | ||||||||||||
| Cholesterol and derivatives | 8.27 | 8.43 | 2.92 | ||||||||||||
| Non-metal oxoanionic compounds | 4.07 | 4.04 | 0.41 | ||||||||||||
| Organic sulfuric acids and derivatives | 23.64 | 25.3 | 27.37 | ||||||||||||
| Organic sulfonic acids and derivatives | 7.08 | 10.9 | 6.10 | ||||||||||||
| Organic carbonic acids and derivatives | 7.67 | 9 | 4.39 | ||||||||||||
| Organic phosphoric acids and derivatives | 8.74 | 8.28 | NA | ||||||||||||
| Benzothiazepines | 22.99 | 22.49 | 2.09 | ||||||||||||
| Bilirubins | 10.37 | 13.28 | NA | ||||||||||||
| Dihydrofurans | 10.09 | 6.84 | 2.42 | ||||||||||||
| Alkyl halides | 7.17 | 5.97 | 2.77 | ||||||||||||
| Sulfinic acids and derivatives | 9.57 | 21.87 | NA | ||||||||||||
| Azoles | 17.19 | 12.88 | 7.62 | ||||||||||||
| Azolidines | 14.11 | 17 | NA | ||||||||||||
| Cinnamic acids and derivatives | 48.32 | 49.73 | NA | ||||||||||||
| Peptidomimetics | 22.76 | 21.34 | NA | ||||||||||||
| Piperidines | 53.36 | 63.22 | NA | ||||||||||||
| Pyrrolidines | 10.55 | 13.2 | NA | ||||||||||||
| Coumarins and derivatives | 30.57 | 17.89 | NA | ||||||||||||
Notes: “High” precision is shown in green (≤10%), “moderate” in yellow (10% < x < 20%), and “low” in red (≥20%).