Table 3.
Reporting Accuracy (%) compared with NIST Metabolites in Frozen Human Plasma (SRM 1950).
| Accuracy (%) | Biocrates | HMT | Nightingale | ||||
|---|---|---|---|---|---|---|---|
| Analyte | NIST Value (uM) | Reported Value (uM) | Percent Difference | Reported Value (uM) | Percent Difference | Reported Value (uM) | Percent Difference |
| Fatty Acids | |||||||
| C18:2 n-6 (Z,Z)-9,12-Octadecadienoic Acid (Linoleic Acid) | 2838 | 2960 | 4.30% | ||||
| C22:6 n-3. (Z,Z,Z,Z,Z,Z)-4,7,10,13,16,19-Docosahexaenoic Acid (DHA) | 118 | 136 | 15.25% | ||||
| Amino Acids | |||||||
| Alanine | 300 | 331 | 10.17% | 211 | −29.61% | 312.246 | 4.08% |
| Glycine | 245 | 288 | 17.72% | 250 | 1.97% | 240.87 | −1.69% |
| Histidine | 72.6 | 80 | 10.08% | 59 | −18.25% | 70.0707 | −3.48% |
| Isoleucine | 55.5 | 66 | 18.92% | 46 | −17.02% | 44.604 | −19.63% |
| Leucine | 100.4 | 114 | 13.05% | 102 | 1.25% | 87.7893 | −12.56% |
| Lysine | 140 | 151 | 7.73% | 129 | −7.60% | ||
| Methionine | 22.3 | 22 | −0.94% | 14 | −38.50% | ||
| Proline | 177 | 199 | 12.30% | 138 | −22.19% | ||
| Serine | 95.9 | 98 | 2.14% | 69 | −27.57% | ||
| Threonine | 119.5 | 127 | 6.10% | 92 | −23.08% | ||
| Tyrosine | 57.3 | 61 | 6.48% | 49 | −13.95% | 61.8318 | 7.91% |
| Valine | 182.2 | 174 | −4.40% | 152 | −16.30% | 185.06 | 1.57% |
| Arginine | 81.4 | 95 | 16.45% | ||||
| Cysteine | 44.3 | 46 | 4.50% | ||||
| Cystine | 7.8 | 8.0 | 2.76% | ||||
| Phenylalanine | 51 | 57 | 12.54% | 47 | −8.27% | 53.0223 | 3.97% |
| Clinical Markers | |||||||
| Creatinine | 60 | 65 | 7.69% | 43 | −28.14% | 58.1642 | −3.06% |
| Glucose | 4560 | 4679.41 | 2.62% | ||||
| Homocysteine | 8.5 | 8.5 | 0.58% | ||||
| Cortisol | 0.23 | 0.19 | −17.92% | ||||
| Cholesterol | 3917 | 3620 | −7.58% | ||||
Notes: “High” accuracy is shown in green (≤10%), “moderate” in yellow (10% < x < 20%), and “low” in red (≥20%). Accuracy assessed only for vendors that reported quantitative units and not relative units; accuracy estimated using Shipment 1 data. All percent accuracy values are versus NIST COA values, such that a negative value is below the NIST-provided reference value.