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. Author manuscript; available in PMC: 2021 Sep 26.
Published in final edited form as: Methods Mol Biol. 2019;1860:211–220. doi: 10.1007/978-1-4939-8760-3_13

Fig. 1.

Fig. 1

Sec18 preferentially binds to liposomes containing PA. Recombinant His6-Sec18 (2 μg) was incubated with liposomes of the indicated compositions for 10 min at 30 °C. Liposomes were isolated by centrifugation and washed before bound proteins were resolved by SDS-PAGE. Bar graphs show average normalized densitometry values measured for three separate experiments. Binding was observed in liposomes with increasing concentrations of PA or PS (a) and with no liposomes (b). *p < 0.05; **p < 0.001; ***p < 0.0001. (Reproduced from [15] with permission from John Wiley and Sons)