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. 2021 Sep 21;10(9):2499. doi: 10.3390/cells10092499

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Induction of lipid accumulation and lipid measurement in meat buds. (a) Scheme of CD29+ cells with adipogenic differentiation. (b,c) Spheroids prepared from Ha2/5+ cells were differentiated into adipose differentiation medium for two weeks after five days of differentiation into muscle. Control spheroid (b) and spheroids induced to adipose differentiation (c) are shown. Desmin (red), BODIPY (green), and Hoechst (Blue). The scale bar represents 100 μm. (d) Relative expression of bovine meat bud by real-time RT-PCR. RNA was extracted from adipose-differentiated bovine meat buds (two weeks) for gene expression analysis of adipose differentiation markers (CEBPα, CEBPβ, PPARγ, LEPTIN, and ADIPONECTIN), and (e) muscle markers (DESMIN and PAX7). (n = 4), * p < 0.05.