Figure 1.

Expression of epithelial and mesenchymal markers in PDAC-derived tumor cell lines as measured by immunoblot analysis. (A) The indicated PDAC cell lines, and HPDE6c7 cells as control, were immunoblotted for ECAD and VIM. Equal loading was verified by detection of β-actin. The blot was overexposed to also catch the weak ECAD band in PANC-1 and the lack of VIM signals in HPDE6c7 and BxPC-3 cells. For a short exposure copy of this blot see Figure S1A. (B) As in (A) except that additional PDAC cell lines were analyzed for ECAD, VIM, CLDN4. Detection of GAPDH served as a loading control. In lysates from COLO 357 cells a band for VIM becomes visible upon longer exposure of the blot (not shown).