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. 2021 Sep 17;13(18):4663. doi: 10.3390/cancers13184663

Figure 5.

Figure 5

Comparison of the invasive and β cell TD potential of COLO 357 and L3.6pl cells. (A) Real-time cell migration assay with COLO 357 and L3.6pl cells. Data are the mean ± SD of quadruplicate wells and are significantly different between cell lines at 6:45 (denoted by an asterisk) and all later time points. The assay shown is representative of three independent RTCA assays. (B) COLO 357 and L3.6pl cells were cultured with P2 (TDC-P2), or a combination of IGF-1, SCF and transferrin (TDC-P3), for 72 h and assayed for INS and SLC2A2 mRNA abundance by qPCR. The data shown are from a representative experiment out of three experiments performed in total (mean ± SD of triplicate wells). Displayed are the values for TDC in relation to those of COLO 357 cells with control medium. The asterisks (*) denote significance (p < 0.05, unpaired two-tailed Student’s t test). The changes in INS and SLC2A2 RNA expression between COLO 357 cells treated with either TDC-2 or TDC-3 and the respective untreated controls were also significant (not shown for reasons of clarity).