Figure 1.

The effects of different concentrations of heparin, enoxaparin and MgCl₂/MgSO₄ on human adenovirus type 5 (HAdV5). Virus and further components were mixed pre-transduction and added to CHO-K1 cells. The luciferase measurements were performed 26 h post-transduction. All experiments included a positive control referring to untreated HAdV5: (a) CHO-K1 cells were infected with 1000 viral particles per cell with HAdV5 in the presence of different international units (I.U.) of heparin and enoxaparin; (b) Transduction rates of HAdV5 in the presence of increasing amounts of MgCl₂; (c) Infectivity of HAdV5 at 1000 viral particles per cell in the presence of different amounts of heparin and 5 µmol of MgCl₂; (d) Effects of MgCl₂ and MgSO₄ on HAdV5 transduction rates. Experiments were performed in triplicates and repeated three times. The mean standard errors are shown.