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. 2021 Sep 10;22(18):9787. doi: 10.3390/ijms22189787

Table 3.

Relative gene expression analysis of 20 randomly selected genes for making the comparison of pl and WT group conducted by RNA- seq and qRT PCR.

Gene ID Gene Name * pl vs. WT Gene Description Pathway/Function Involved
RNA-seq log2 fold change qRT PCR fold change
Os02g0626100 OsPAL1 3.19286832 3.01 ± 0.01 Similar to Phenylalanine ammonia-lyase Anthocyanin Biosynthesis
Os02g0626400 OsPAL2 2.10996231 1.93 ± 0.03 Phenylalanine ammonia-lyase Phenylpropanoid metabolism, Anthocyanin Biosynthesis
Os04g0518400 OsPAL6 5.02738052 3.10 ± 0.19 Similar to Phenylalanine ammonia-lyase Phenylpropanoid metabolism, Anthocyanin Biosynthesis
Os05g0427400 OsPAL7 3.03883343 1.25 ± 0.10 Similar to Phenylalanine ammonia-lyase Phenylpropanoid metabolism
Os02g0177600 Os4CL3 1.82828648 1.19 ± 0.10 4-coumarate: coenzyme A ligase Phenylpropanoid metabolism
Os08g0448000 Os4CL5 4.87114854 2.08 ± 0.14 4-coumarate: coenzyme A ligase 5 Phenylpropanoid metabolism
Os03g0122300 OsF3H 4.76468487 2.37 ± 0.24 flavonol synthase Flavonoid biosynthesis, Anthocyanin Biosynthesis
Os08g0441500 4.4886503 3.93 ± 0.015 cinnamoyl-CoA reductase Phenylpropanoid metabolism, Anthocyanin Biosynthesis
Os05g0320700 1.8282864 1.65 ± 0.31 cinnamate 4-hydroxylase 2 Phenylpropanoid metabolism, Anthocyanin Biosynthesis
Os10g0512400 OsCAld5H1; F5H1 3.1612775 2.57 ± 0.15 coniferaldehyde 5-hydroxylase 1, ferulate 5-hydroxylase 1 Lignin biosynthesis
Os01g0196300 DPF; OsbHLH025 5.6010349 3.65 ± 0.020 basic helix-loop-helix protein 025
Os01g0838350 Phosphate-limitation inducible gene 1 −1.0446037 −0.59 ± 0.09 Conserved hypothetical protein
Os01g0734800 1.120071 1.65 ± 0.10 anthocyanidin 5,3-O-glucosyltransferase Anthocyanin Biosynthesis
Os11g0116300 2.70218828 2.25 ± 0.02 chalcone isomerase Anthocyanin Biosynthesis
Os02g0611800 2.38197885 1.26 ± 0.08 Hydroxycinnamoyltransferase 2 Phenylpropanoid and Flavonoid biosynthesis
Os02g0194700 OsLOX1 −2.9658063 −0.68 ± 0.06 Lipoxygenase 1
Os11g0610700 OsWD40-190 1.2940596 1.71 ± 0.10 WD40 repeat-like domain containing protein
Os07g0543100 −2.9658063 −0.30 ± 0.01 Beta-amylase 1
Os02g0738100 3.24756852 1.19 ± 0.13 basic helix-loop-helix protein
Os04g0662600 4.492093 1.66 ± 0.07 Flavanone 3-hydroxylase 1 Flavonoid biosynthesis

* Gene IDs are arranged following the gene order of the anthocyanin biosynthetic pathway.