Figure 2.

(A) Representative histograms of cell cycle analysis after exposure to LyeTx I-b and carboplatin at IC₅₀ values for 48 h. The results represent three independent experiments in triplicate and show significant differences between the negative control and the treated cells in terms of the G0/G1 (sub-diploid) population. (B) Illustrative histogram representing the quantification of the proportions of the dead and live cells after exposure of 4T1 cells to 6.5 µM of LyeTx I-b for 24 h and staining with annexin V/Propidium iodide (PI). The number of cells in each quadrant (Q) gate can be quantified as follows: Q4 = live cells (PI negative/annexin V negative); Q3 = apoptotic cells (PI negative/annexin V positive); Q2 = necrotic or late apoptotic cells (PI positive/annexin V positive); and, eventually, Q1 = nuclei without plasma membrane = (PI positive/annexin V negative). Three independent experiments in triplicate and unpaired t-test were performed, ** p < 0.01; *** p < 0.001 compared to the control group.