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. 2021 Aug 27;10(9):2218. doi: 10.3390/cells10092218

Figure 5.

Figure 5

1H11 induces ROS production and modulates the expression of critical proliferative and migratory effector proteins. (A) After 72 h of treatment, ROS levels were evaluated by DCFDA staining. 1H11 caused an increase in ROS levels. (B,C) Serum-starved cells were treated for 10 min, and lysates were accessed by Western blotting. 1H11 treated cells showed decreased phosphorylation of (B) JAK2 and (C) STAT3. (D,E) Cells were incubated with 1H11 or IgG for 72 h prior to harvest and evaluation by Western blotting. (D) 1H11 treatment resulted in an increased expression of two G1-checkpoint CDK inhibitors, p27 and p21. (E) An increase in E-cadherin and a decrease in vimentin protein expression were also observed. For Western blotting, GAPDH was used as a loading control. All experiments were performed by treating OVCAR8 cells with 125 nM 1H11 or IgG. All data plotted as mean ± SEM. *** p < 0.001, ** p < 0.01 vs. IgG. (F) Proposed molecular mechanism of 1H11.