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. 2021 Aug 24;10(9):1334. doi: 10.3390/antiox10091334

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of compounds 7–9 on p38, JNK-1/2, and ERK-1/2 phosphorylation in BV2 (A) and RAW264.7 (B) cells. Cells were pretreated with indicated concentrations of compounds 7–9 for 3 h and stimulated for 30 min with LPS (1 μg/mL). Cell extracts were analyzed using Western blotting with antibodies specific for phosphorylated p-p38, p-JNK1/2, and p-ERK1/2. Membranes were stripped and re-probed to measure total abundance of each mitogen-activated protein kinase (MAPK) as a control measurement. Representative blots from three independent experiments are shown.