Figure 3.

Wild-type dSesn expressed in muscle of dSesn^−/− flies improves climbing speed and increases fat body lysosomal activity. Wild-type, C86S, D423A, D423A/D424A dSesn transgenes were expressed in muscle of dSesn^−/− flies. (A,B) dSesn^−/−;Mef2 > dSesn^WT climb faster than dSesn^−/−;Mef2 > dSesn^CS (shown in (A)), dSesn^−/−;Mef2 > dSesn^DA and dSesn^−/−;Mef2 > dSesn^DDAA flies (shown in (B)) whether exercised or not (2-way ANOVA, genotype effect, dSesn^−/−;Mef2 > dSesn^WT compared to unexercised and exercised Mef2 > dSesn^CS, dSesn^−/−;Mef2 > dSesn^DA and dSesn^−/−;Mef2>dSesn^DDAA f lies: p ≤ 0.0335, d15+). Climbing speed experiments performed in triplicate, n ≥ 100 flies. (C,D) dSesn^−/−;Mef2>dSesn^WT flies have higher Lysotracker staining in fat bodies than dSesn^−/−;Mef2 > dSesn^CS (shown in (C), compare to red bars), dSesn^−/−;Mef2 > dSesn^DA and dSesn^−/−;Mef2 > dSesn^DDAA flies (shown in (D), compare to red bars in (C)) (ANOVA, p ≤ 0.0319). Exercise increases fat body Lysotracker in dSesn^−/−;Mef2 > dSesn^CS (shown in C) (ANOVA, p < 0.0001) (E) Exercised dSesn^−/−;Mef2 > dSesn^CSflies have lower myocardial Lysotracker than dSesn^−/−;Mef2 > dSesn^WT flies (ANOVA, p ≤ 0.0102). (F) Myocardial Lysotracker staining in dSesn^−/−;Mef2 > dSesn^DA and dSesn^−/−;Mef2 > dSesn^DDAA flies is similar to flies expressing Wild-type dSesn (Compare to red bars in (E)) whether exercised or not (ANOVA, p ≥ 0.3075). Lysotracker staining n = 5, performed in duplicate or triplicate. Brackets represent pairwise post-hoc comparisons from ANOVA. Error bars depict ± SD. EX = cohort after 3 weeks of ramped, daily endurance training. UN = control unexercised flies exposed to identical training environment without running. ns or lack of brackets indicates no significant difference.