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. 2021 Sep 19;22(18):10120. doi: 10.3390/ijms221810120

Figure 2.

Figure 2

Kisspeptin system in dog sperm and epididymis fluid. (A) Representative Western blot of Kiss1R and Kiss1 carried out on total protein extracts from sperm collected at different tracts of the epididymis. (B) Protein levels were normalized against GAPDH (upper panel) and then against the SPZ count (lower panel) and data were reported as Kiss1R/GAPDH ± S.D (upper panel) and normalized Kiss1R/SPZ number (106/mL) ± S.D (low panel) (N = 4). (C) Western blot for Kiss1R carried out on crude membrane fraction (N = 3). (D) Representative dot blot for Kiss1 carried out on epididymis fluid and spermatozoa-free seminal plasma (N = 4). BSA = bovine serum albumin; dT= dog testis, EH: epididymal head sperm; EC1: sperm from the cranial portion of epididymal corpus; EC2: sperm from the caudal portion of the epididymal corpus; ET: epididymal tail sperm; EFH: epididymis fluid from the epididymal head; EFC1: epididymis fluid from the cranial portion of the epididymal corpus; EFC2: epididymis fluid from the caudal portion of the epididymal corpus; EFT: epididymis fluid from the epididymal tail; M = protein ladder; SP: seminal plasma without spermatozoa. Different letters indicate statistically significant differences (p < 0.05).