Fig. 2.

MDA-MB-468 cells are resistant to JL5 and sensitive to Ym155. A Cell counts of MDA-MB-468 cells treated for 24 h with JL5 and Ym155 alone and in combination. B–G Western blot analysis of Id1, XIAP, BMPR2 in MDA-MB-468 cells treated for 24 h and densitometric analysis of expression relative to loading control. H Immunoblot for BMPR2 of untreated cell lines. I–J Representative immunofluorescent images of BMPR2 at the plasma membrane and in the cytosol in the different cell lines. K Quantification of the number of cells that expressed BMPR2 at the plasma membrane. Approximately 50 cells from each cell line were examined for BMPR2 expression on the plasma membrane. Data is reported as the mean number of cells expressing BMPR2 at the plasma membrane. Each scale bar represents 10 μM