Fig. 7.

JL5 and Ym155 activation of the lysosomes regulates BMPR2 degradation and lysosomal mediated cell death. A Western blot analysis for BMPR2 with JL5 combined with Ym155 with and without VAD and chloroquine. B Densitometric analysis of BMPR2 relative to GAPDH expression. C Immunofluorescence imaging for Green Cathepsin B of A549 cells treated with chloroquine (chloro) and VAD alone and in combination. D, E Immunofluorescence imaging for BMPR2 at the plasma membrane of H1299 cells treated for 24 h with JL5 in combination with Ym155 with and without VAD + chloroquine. F Western blot of MDA-MB-468 cells treated with Ym155 with and without chloroquine and G densitometric analysis of expression relative to loading control. H Immunonofluorescence imaging of BMPR2 at the plasma membrane treated with chloroquine for 24 h. I, J Immunofluorescence imaging for Green Cathepsin B of H1299 and A549 cells treated with JL5 + Ym155 for 24 h. K, L Cell counts demonstrating percent dead cells of cells treated for 24 h with and without lysosome inhibitors. Cell count data represents the mean of 4 independent experiments. *represents p < .05 compared to vehicle controls. Each scale bar represents 10 μM