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. 2021 Sep 25;20:380. doi: 10.1186/s12936-021-03894-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Serial dilutions of patient ID 2 for the determination of the LOD in patient samples using 18 s primers. A A representative gel from dilutions of ID 2 with concentrations ranging from 12.6 to 0.0126 parasites/μL. Only the 12.6 parasites/μL sample showed consistent amplification in gel represented by the dark smeared banding. B Diffusion coefficients from smartphone PD analysis of diluted samples yielded a LOD of 0.126 parasites/µL blood. All dilutions, 12.6 parasites/µL blood (****p < 0.0001), 1.26 and 0.126 parasites/µL blood (*p < 0.05), except 0.0126 parasites/µL blood were found to be statistically different from NTC. (N = 3)