Figure 4.

The effect of HY7715 treatment on myogenic development and differentiation in aged mice. (A) Hematoxylin and eosin (H&E) staining of limb muscle fiber after 5 weeks of treatment. (B) The cross-sectional area (CSA) of muscle fibers were quantified. The mRNA level of MyoD and MHY1 in gastrocnemius muscle (C,D) and soleus muscle (E,F) were normalized to the level of GAPDH mRNA and calculated as a relative-fold value. Statistical significance was determined using one-way ANOVA followed by Tukey’s post hoc test (N = 5). Datasets denoted by different letters are significantly different; p < 0.05 (a > b > c). YM, young mice; OM, old mice; OM + C, creatine-treated old mice; OM + HY, HY7715-treated old mice.