Figure 5.

The effect of HY7715 treatment on age-induced sarcopenic factors in mice. (A) The plasma level of TNFα in mice was measured using a commercial colorimetric enzyme-linked immunosorbent assay (ELISA) kit. (B) Western blot analysis of myostatin and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in gastrocnemius muscle of the mice. The mRNA levels of muscle atrophy F-box gene (Atrogin1) and muscle RING-finger protein 1 (MuRf1) in gastrocnemius muscle. (C) The protein level of myostatin was quantified. The mRNA level of Atrogin1 and MuRf1 in gastrocnemius muscle (D,E) and soleus muscle (F,G) were normalized to the level of GAPDH mRNA and calculated as a relative-fold value. Statistical significance was determined using one-way ANOVA followed by Tukey’s post hoc test (N = 5). Datasets denoted by different letters are significantly different; p < 0.05 (a > b > c). YM, young mice; OM, old mice; OM + C, creatine-treated old mice; OM + HY, HY7715-treated old mice.