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. 2021 Sep 16;22(18):10023. doi: 10.3390/ijms221810023

Figure 6.

Figure 6

The effect of HY7715 treatment on energy metabolism factors in mice. (A) The mRNA level of transcription factor A, mitochondrial (TFAM1), and uncoupling protein 3 (UCP3) in gastrocnemius muscle (A,B) and soleus muscle (C,D) were normalized to the level of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA and calculated as a relative-fold value. (E) Western blot analysis of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1α) and GAPDH in gastrocnemius muscle from the mice. (F) The protein level of PGC1α was quantified. Statistical significance was determined using one-way ANOVA followed by Tukey’s post hoc test (N = 5). Datasets denoted by different letters are significantly different; p < 0.05 (a > b > c). YM, young mice; OM, old mice; OM + C, creatine-treated old mice; OM + HY, HY7715-treated old mice.