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. 2021 Sep 16;22(18):10023. doi: 10.3390/ijms221810023

Figure 8.

Figure 8

The effect of different cell fractions of HY7715 on C2C12 myoblast differentiation. (A) An MTT assay in the C2C12 cell line. The mRNA level of (B) tumor necrosis factor-α (TNFα) and (C) muscle atrophy F-box gene (Atrogin1) in C2C12 cells treated with HY7715 cell fractions were normalized to the level of GAPDH mRNA and calculated as a relative-fold value. (D) Adenosine triphosphate (ATP) production was detected using a commercial colorimetric enzyme-linked immunosorbent assay (ELISA) kit. The mRNA level of (E) myoblast determination protein 1 (MyoD) and (F) myogenin in C2C12 cells treated with HY7715 cell fractions were normalized to the level of GAPDH mRNA and calculated as a relative-fold value. Statistical significance was determined using one-way ANOVA followed by Tukey’s post hoc test (N = 5). Datasets denoted by different letters are significantly different; p < 0.05 (a > b > c > d). CON, control cells; Live: live whole cells of HY7715; TL, total lysates of heat-killed HY7715; PL, pellet of lysates of heat-killed HY7715; SL, supernatant of lysates of heat-killed HY7715; TNFα, 100 ng/mL TNFα-treated cells; TNFα + TL; total lysates of heat-killed HY7715 and 100 ng/mL TNFα-treated cells.