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Multiple sequential injections—high performance countercurrent chromatography (HPCCC) method to obtain fucoxanthin from Phaeodactylum tricornutum extract. Biphasic solvent system: System 1, mixture of n-heptane, ethyl acetate, ethanol and water (ratio 5:5:6:3, v/v/v/v). Sample loading: 240 mg of extract dissolved in 3 mL of mobile phase (3 mL sample loop). Runs: 10 consecutive injections. Flow rate: 4 mL/min. Rotational speed: 1600 rpm. Column temperature: 30 °C. Detection: 450 nm.
