Table 1.
The partition coefficient (K) of fucoxanthin in different biphasic solvent systems, density differences and the settling times.
| Solvent Systems | Composition | Relative Proportions of Solvents (v/v/v/v) | Phase Volume Ratio (UP/LP) | Settling Time (s) | Density Difference (LP−UP, g/mL) | Partition Coefficient (K) of Fucoxanthin |
|---|---|---|---|---|---|---|
| 1 | n-Hep–EtoAc–EtOH–H2O | 5/5/6/3 | 0.58 | 17 | 0.1284 | 0.515 |
| 2 | n-Hep–EtoAc–EtOH–H2O | 5/5/7/3 | 0.43 | 15 | 0.1171 | 0.314 |
| 3 | n-Hep–EtoAc–EtOH–H2O | 5/5/8/3 | 0.38 | 18 | 0.1311 | 0.205 |
| 4 | n-Hep–EtoAc–EtOH–H2O | 5/5/6/4 | 0.59 | 18 | 0.1301 | 0.897 |
| 5 | n-Hep–EtoAc–EtOH–H2O | 5/5/6/5 | 0.59 | 20 | 0.1497 | 1.942 |
| 6 | n-Hep–EtoAc–EtOH–H2O | 5/5/5/3 | 0.80 | 20 | 0.1232 | 0.590 |
LP: Lower phase. UP: Upper phase.