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. 2021 Sep 16;13(18):4645. doi: 10.3390/cancers13184645

Figure 2.

Figure 2

QC impairs RBG and induces nucleolar stress in OC cells. (A) Graphic representation of 47S pre-ribosomal RNA. ETS–external transcribed spacers, ITS–internal transcribed spacers. (B) Relative expression changes for ribosomal subunits after 1 h of 1 µM QC treatment in OVCAR5 and (C) OVCAR8 cells (normalized to RPLP0; n = 3). Error bars represent standard error, and significance is denoted by ** (p < 0.01). (D) Incorporation of 5-fluorouridine was monitored after 10 min of labeling in OVCAR5 cells treated with 1 µM QC for 2 h. Representative images were shown, quantified and shown as bar graph **** (p < 0.0001). (E) Fibrillarin (Green) IF staining was performed after 2 h of 1 µM QC in OVCAR8 cells. DAPI was used to stain nucleus. (F) Whole cell lysates of OVCAR5 were analyzed for puromycin incorporation (1 h, 10 µM) with increasing QC concentrations. Membrane with Ponceau stain was shown for protein loading. Abbreviations: QC: Quinacrine; ITS1/2: internal transcribed spacers 1 and 2; 5′/3′ ETS: external transcribed spacers; FUrd: 5-fluorouridine.