Figure 8.

Promoter activities of Ren (A) and Cd38 (B) in the As4.1 JG cells. Reporter plasmids prepared by inserting the promoter fragments of Ren (−4094 ~ +33) and Cd38 (−4888 ~ +92) upstream of a firefly luciferase reporter gene in a pGL4.17 vector were transfected into the As4.1 cells. After the cells were exposed to either IH or normoxia for 24 h, they were lysed, and the promoter activities of Ren and Cd38 were measured. All the data are represented as the mean ± SE of the samples of six independent experiments (n = 6). The statistical analyses were performed using a Student’s t-test.