Figure 4.

Relative growth of wild-type and mutant cells in batch cultures in the presence of sodium chloride. Wild-type (WT) and mutant cells (∆RTG2, ∆HOG1, ∆HOG1∆RTG2 and ∆RTG3), grown overnight in YPD medium, were diluted to 0.1 OD₆₀₀ in fresh liquid YPD with or without 0.8 M sodium chloride (NaCl), and optical density (OD₆₀₀) was measured at 600 nm at the indicated times. Relative growth was calculated as the percentage of the OD₆₀₀ of stressed/control cells. Unpaired Student’s t-test: statistical significance differences with ** p < 0.01 and * p < 0.05 when comparing wild type with RTG mutants from six independent experiments at 24 h.