Figure 5.

CIT2 expression under high osmotic environment. Wild-type (WT) and ∆RTG2 cells, grown overnight in YPD medium, were diluted to 0.1 OD₆₀₀ in fresh liquid YPD with or without 0.8 M sodium chloride (NaCl). After 5 h, cells were collected for RNA extraction, and mRNA levels were measured by quantitative PCR. The amount of CIT2 mRNA was normalized with ACT1 mRNA and calculated in relative units (2^−ΔCt), where ΔCt is the Ct_sample−Ct_reference gene and Ct is the threshold cycle. Unpaired Student’s t-test: a statistically significantly difference with ** p < 0.01 when comparing wild-type untreated cells versus NaCl-stressed cells from three independent experiments.