Table 4.

Overview of the costs for the different methods. These costs only include running costs, such as consumables, fee for using microscopes and working hours, but no initial set up costs (e.g., purchase of equipment) and neither costs on the side from clinics or patients for the visits (of which more are needed if only fresh material is used).

Investigation/Method	Consumables and Fee for Equipment Use	Working Hours	Total
nasal brushing	EUR 6	EUR 70	EUR 76
cell culture	EUR 250	EUR 260	EUR 510
HSVM fresh	EUR 24	EUR 180	EUR 204
HSVM ALI	EUR 24	EUR 140	EUR 164
IF fresh 1	EUR 155	EUR 170	EUR 325
IF ALI 1	EUR 155	EUR 120	EUR 275
TEM fresh	EUR 500	EUR 1500	EUR 2000
TEM ALI	EUR 250	EUR 750	EUR 1000
Reporting/meetings/etc.	EUR 0	EUR 181	EUR 181
Genetics 2	EUR 200	EUR 2800	EUR 3000
all standard methods 3	EUR 459	EUR 770	EUR 1229
all methods 4	EUR 909	EUR 4501	EUR 5410

Remark about costs: PCD-UNIBE is based in Switzerland where salaries and prices for consumables are usually higher than in other European countries. Original costs were calculated in Swiss francs. For the conversion to Euros, an exchange rate of 1.1 CHF per one EUR was used. ¹ These costs apply for the standard panel including DNAH5, GAS8, and RSPH9. Costs are higher if additional proteins are stained. ² Average costs, costs vary depending on affected gene. ³ Standard methods include nasal brushing, cell culture, HSVM fresh, HSVM ALI, and IF ALI. ⁴ All methods include nasal brushing, cell culture, HSVM fresh and ALI, IF ALI, TEM ALI, and genetics.