Table 2.
Summary of the cell-type-specific differences involving bioenergetics, relative expression of SDHs, SDHs-multiprotein assembly, and PTMs of SDHs observed under CAMKK2 deletion conditions in HEK293 and HepG2 cells
| Cell line | HEK293 | HepG2 |
|---|---|---|
| Effect of CAMKK2 deletion | ||
| Tissue source | Embryonic kidney | Liver |
| Origin/disease | Transformed cell line | Hepatocellular carcinoma |
| Morphology | Epithelial | Epithelial-like |
| Basal cellular respiration | Decreased | Decreased |
| CI-IV respiration | Increased | Decreased |
| CII-IV respiration | Increased | Decreased |
| SDHA mRNA level | Decreased | Unaltered |
| SDHB mRNA level | Increased | Decreased |
| SDHC mRNA level | Unaltered | Unaltered |
| SDHA protein level | Increased | Unaltered |
| SDHB protein level | Increased | Decreased |
| SDHC protein level | Increased | Unaltered |
| SDHB-associated MPC | Shifted to the higher mol. weight | Shifted to the lower mol. weight |
| SDHB PTM state | Charged fractions altered | Charged fractions unaltered |
| SDH enzymatic activity | Increased | Decreased |