Figure 7.

Infliction of burn wounds causes an inflammatory response in reconstructed human epidermis. Concentrations of inflammatory cytokines IL-8, IL-6, IL-1β, and VEGF in cell culture supernatants of burned and unwounded models at distinct time points over 14-day period determined by CBA. The detected IL-8 and VEGF levels peaked 3 h after burning. IL-6 and IL-1β were very low at any given time point (3 biological replicates in independent test runs with 3 technical replicates each; mean values ± SD; Kruskal-Wallis test with Dunn’s multiple comparisons test, * p < 0.5, ** p < 0.01, *** p < 0.001, compared to the control; ° p < 0.05, compared to burned models).