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. 2021 Sep 17;22(18):10039. doi: 10.3390/ijms221810039

Figure 5.

Figure 5

Expression of TTG1 under the control of its native promoter restores secondary metabolism in the ttg1Δ15aa mutant. (a) Seed coat color in the Col wild type, the ttg1Δ15aa mutant and the TTG1/ttg1Δ15aa transgenic plants. Pictures were taken under a Motic K microscope by using an EOS 1100D digital camera. (b) Mucilage production in the Col wild type, the ttg1Δ15aa mutant, and the TTG1/ttg1Δ15aa transgenic plants. Seeds were stained with 0.01% (w/v) Ruthenium red for 2 h, and pictures were taken under a Motic K microscope by using an EOS 1100D digital camera. (c) Anthocyanin accumulation in seedlings of the Col wild type, the ttg1Δ15aa mutant, and the TTG1/ttg1Δ15aa transgenic plants in response to sucrose. Seeds of Col wild type, the ttg1Δ15aa mutant, and the TTG1/ttg1Δ15aa transgenic plants were germinated and grown on half MS plates with or without 3% sucrose. Pictures were taken from 6-day-old seedlings.