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. 2021 Sep 17;22(18):10039. doi: 10.3390/ijms221810039

Figure 6.

Figure 6

Interaction of full-length and truncated TTG1 with GL3. (a) Interaction of TTG1, TTG1Δ15aa, TTG1Δ10aa, TTG1Δ5aa, TTG1Δ4aa, TTG1Δ3aa, TTG1Δ2aa, TTG1Δ1aa, and TTG1Δ339L with GL3 in transfected protoplasts. Protoplasts were isolated from 3 to 4-week-old Col Arabidopsis rosette leaves. Plasmids of the effector genes GD-TTG1, GD-TTG1Δ15aa, GD-TTG1Δ10aa, GD-TTG1Δ5aa, GD-TTG1Δ4aa, GD-TTG1Δ3aa, GD-TTG1Δ2aa, GD-TTG1Δ1aa, or GD-TTG1Δ339L were co-transfected with the reporter gene Gal4:GUS and the effector gene HA-GL3 into protoplasts. The protoplasts were incubated in darkness for 20–22 h, and then GUS activity was assayed. Co-transfection of GD with Gal4:GUS and HA-GL3 was used as a control. Data represents the mean ± SD of three repeats. *, Significantly different from that in the GD control (Student’s t test, p < 0.0001). (b) Alignment of the C-terminal amino acids of TTG1, BraTTG1, GmTTG1, PtrTTG1, OsTTG1, ZmTTG1, AtLWD1, and AtLWD2 proteins. Identical amino acids are shaded in black, and similar in grey.