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. 2021 Sep 17;22(18):10039. doi: 10.3390/ijms221810039

Figure 8.

Figure 8

The expression of TTG1Δ3aa under the control of TTG1 native promoter failed to restore trichome formation in the ttg1Δ15aa mutant. (a) Trichome formation on leaves of 10-day-old seedlings (up panel), 4-week-old plants (middle panel), and stems of 5-week-old plants (lower panel) of the Col wild type, the ttg1Δ15aa mutant, and the TTG1Δ3aa/ttg1Δ15aa transgenic plants. Seeds of Col wild type, the ttg1Δ15aa mutant, and the TTG1Δ3aa/ttg1Δ15aa transgenic plants were germinated and grown in soil pots. Pictures were taken directly or under a Motic K microscope by using an EOS 1100D digital camera at indicated stages. (b) Expression of TTG1Δ3aa in Col wild type, the ttg1Δ15aa mutant, and the TTG1Δ3aa/ttg1Δ15aa transgenic plants. RNA was isolated from 10-day-old seedlings and qRT-PCR was used to examine the expression of TTG1Δ3aa. The expression of ACT2 was used as an inner control. The expression level of TTG1Δ3aa in the Col wild type was set as 1. Data represent the mean ± SD of three replicates. *, Significantly different from that in the Col wild type (Student’s t test, p < 0.01). (c) Expression of TTG1 in Col wild type, the ttg1Δ15aa mutant, and the TTG1/ttg1Δ15aa transgenic plants. RNA was isolated from 10-day-old seedlings, and qRT-PCR was used to examine the expression of TTG1. The expression of ACT2 was used as an inner control. The expression level of TTG1 in the Col wild type was set as 1. Data represent the mean ± SD of three replicates. *, Significantly different from that in the Col wild type (Student’s t test, p < 0.01).