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. 2021 Aug 28;9(9):1106. doi: 10.3390/biomedicines9091106

Figure 1.

Figure 1

Expression and purification of the anti-ErbB3 single-domain antibodies and ErbB3 receptor fragments. (a) Scheme of SUMO fusions used to produce single-domain antibodies; (b) SDS-PAGE and Western blot of soluble extracts, the recombinant fusion proteins have MW 27 kDa; BCD090-M2 expressed in soluble form only in SHuffle cells, but not BL21(DE3); (c) SDS-PAGE of the purified single-domain antibodies, periplasmically expressed pelB-BCD090-P1-His5 migrates higher due to the presence of uncleaved pelB leader and histidine tag; (d) SDS-PAGE of the purified human ErbB3 extracellular domain (ECD, MW 75 kDa) and the extracellular subdomain 3 (ECDIII, MW ~25 kDa); receptor fragments analyzed in the native glycosylated form and after treatment with PNGase F.