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Evaluation of PD-L1 expression levels in breast cancer cell lines. PD-L1 in situ mRNA and protein expression using RNAscope® and IF, respectively, in MCF7 (A) and BT549 (B) cells. Simultaneous detection of PD-L1 protein and mRNA expression using sequential RNA-FISH/IF method in MDA-MB-231 BC cell line (C); original magnification: 630×.
