Hematological response of O. mykiss fed with five experimental diets. Red blood cell (RBC) count, packed cell volume (PCV) (A), and insulin-like growth factor-1 (IGF-1) level (B) in O. mykiss blood serum were measured. HIL0, 0% HIL; HI25, 25% normal HIL inclusion as fishmeal weight basis; HIL50, 50% normal HIL inclusion as fishmeal weight basis; ImHIL25, 25% immunized HIL inclusion as fishmeal weight basis; ImHIL50, 50% immunized HIL inclusion as fishmeal weight basis. Values were taken as a mean of triplicate analyses. Vertical error bars represent standard error of the mean, and lowercase alphabetic characters represent significant differences (p < 0.05) among groups at each week 3 and week 6 as determined by Duncan’s multiple range test, respectively (n = 9, 3 tanks, 3 fish per tank).