Figure 3.

Measuring immunity-enhancing degree in rainbow trout fed with imHIL-containing feed. Aspartate aminotransferase (AST) (A), lysozyme activity (B), and immunoglobulin M (IgM) (C) were quantitatively analyzed. Initial AST level of rainbow trout blood serum was 67.57 ± 16.72 U L⁻¹. Values are means ± standard deviations of triplicates. HIL0, 0% HIL; HI25, 25% normal HIL inclusion as fishmeal weight basis; HIL50, 50% normal HIL inclusion as fishmeal weight basis; ImHIL25, 25% immunized HIL inclusion as fishmeal weight basis; ImHIL50, 50% immunized HIL inclusion as fishmeal weight basis. Vertical error bars denote standard error of mean, and alphabetic characters represent significant differences (p < 0.05) among each group at week 3 and week 6 as determined by Duncan’s multiple range test, respectively (n = 9, 3 tanks, 3 fish per tank).