Figure 4.

Accumulation of phosphorylated α-syn in mDA neuronal culture from fPD patient. (A) Seventy-day-old DA neuronal cultures from healthy and fPD lines were immunostained with an antibody against phosphorylated α-syn at serine 129 residue, a marker of LBs. The punctated accumulation of pathological α-syn next to the nucleus, resembling LBs, is indicated by an arrow, and diffuse pS129 immunostaining, resembling the early stage of LBs, is indicated by an arrowhead. (B) Levels of pS129 immunostaining were quantified using image analysis. Images are representative of at least three independent differentiations. All data in (B) are presented as mean ± SEM of three images acquired from three different coverslips. Data were analyzed by one-way ANOVA followed by Tukey’s multiple comparison post hoc test. * p < 0.05. The scale bar is 10 µm.