Figure 5.

The effects of PFI-3 on IFN-induced gene expression in GBM cells. (A) RNA was prepared from MT330 cells treated for 6 h with PFI-3 (concentrations indicated) and IFN (1000 IU/mL), and the expression of the indicated genes was determined by qPCR and normalized to actin expression. (B) MT330 and LN229 BRG1^KO cells were transiently co-transfected with IRF-1 luciferase gene reporter construct and Renilla construct, and 24 h after transfection treated with PFI-3 (10 μM)) or IFN (1000 IU/mL) alone or in combination, and then harvested to measure reporter activity. Luciferase activity was normalized to Renilla activity. p values < 0.05 (*), 0.01 (**) and 0.001 (***).