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. 2021 Sep 25;17:313. doi: 10.1186/s12917-021-03025-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — PCR identification of C. jejuni and cpa-positive C.perfringens. For C. jejuni identification A: Lanes 1 and 19, C. jejuni genomic DNA; lanes 2 and 18, 100bp DNA ladder; lanes 3 and 17, no template control; lanes 4, 5, 6, 7, 10, 11, 15 and 16, C. jejuni hipO positive samples; lanes 8, 9, 12, 13 and 14, C. jejuni hipO gene negative samples. For C. perfringens identification. Samples in lanes 1-10 and 11-19 were run on the top and bottom halves of the same gel, respectively. B: Lanes 1 and 20, C. perfringens genomic DNA; lanes 2 and 19, 100bp DNA ladder; lanes 3 and 18, no template control; lanes 4, 5, 6, 7, 8, 9, 10, 11, 12, 13 and 14, 15, 16 and 17, C. perfringens cpa gene positive samples. Lane 15 has a faint band. All samples were tested at least twice. Samples in lanes 1-10 and 11-20 were run on the top and bottom halves of the same gel, respectively