Role of PI3-kinase in insulin-induced GLUT4 translocation in Gαq expressed 3T3-L1 adipocytes. After 60 h of Gαq-expressing adenovirus infection (MOI = 10), 3T3-L1 cells were injected with anti-p110α, anti-p110γ, or sheep IgG as a control (Cont). After insulin stimulation, fixed cells were stained with rabbit anti-GLUT4 antibody and incubated with TRITC-conjugated anti-rabbit IgG antibody and FITC-conjugated anti-goat or anti-sheep IgG antibody, as described in Materials and Methods. The data are means and standard errors from three independent experiments.